European Food Research and Technology
Safety assessment, genetic relatedness and bacteriocin activity of potential probiotic Lactococcus lactis strains from rainbow trout (Oncorhynchus mykiss, Walbaum) and rearing environment
Carlos Araújoa-e, Estefanía Muñoz-Atienzaa, Martha Ramíreza, Patrícia Poetab-c, Gilberto Igrejasd-e, Pablo E. Hernándeza, Carmen Herranza and Luis M. Cintasa*
a Grupo de Seguridad y Calidad de los Alimentos por Bacterias Lácticas, Bacteriocinas y Probióticos (Grupo SEGABALBP), Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040-Madrid, Spain
b Centre for Animal Science and Veterinary, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
c Veterinary Science Department, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
d Institute for Biotechnology and Bioengineering, Centre of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
e Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
* Corresponding author. Phone: +34913943751. Fax: +34913943743. E-mail address: lcintas@vet.ucm.es (L.M. Cintas).
Online Resource 1
Table S1 Oligonucleotide primers used in this study
Table S1 Oligonucleotide primers used in this study
Target gene
|
Primer
|
5’–3’ sequencea
|
PCR fragment size (bp)
|
Annealing temperature (°C)
|
Reference
|
aad(E)
|
aadEI
|
GCAGAACAGGATGAACGTATTCG
|
369
|
55
|
[83]
|
aadEII
|
ATCAGTCGGAACTATGTCCC
|
aph(3′)-IIIa
|
aph(3')-F
|
GCCGATGTGGATTGCGAAAA
|
292
|
60
|
[87]
|
aph(3')-R
|
GCTTGATCCCCAGTAAGTCA
|
lin(A)
|
linA
|
GGTGGCTGGGGGGTAGATGTATTAACTGG
|
323
|
57
|
[85]
|
linA'
|
GCTTCTTTTGAAATACATGGTATTTTTCGATC
|
lin(B)
|
LINB1
|
CCTACCTATTGTTTGTGGAA
|
925
|
54
|
[80]
|
LINB2
|
ATAACGTTACTCTCCTATTC
|
tetB(P)
|
TetB/P-FW
|
AAAACTTATTATATTATAGTG
|
169
|
46
|
[83]
|
TetB/P-RV
|
TGGAGTATCAATAATATTCAC
|
tet(K)
|
tetKI
|
CAATACCTACGATATCTA
|
352
|
50
|
[83]
|
tetKII
|
TTGAGCTGTCTTGGTTCA
|
tet(L)
|
tetLI
|
TGGTCCTATCTTCTACTCATTC
|
385
|
53
|
[83]
|
tetLII
|
TTCCGATTTCGGCAGTAC
|
tet(M)
|
tetMI
|
GGTGAACATCATAGACACGC
|
401
|
45
|
[83]
|
tetMII
|
CTTGTTCGAGTTCCAATGC
|
tet(O)
|
tetOI
|
AGCGTCAAAGGGGAATCACTATCC
|
1,723
|
55
|
[83]
|
tetOII
|
CGGCGGGGTTGGCAAATA
|
tet(Q)
|
TetQ-FW
|
AGAATCTGCTGTTTGCCAGTG
|
169
|
63
|
[83]
|
TetQ-RV
|
CGGAGTGTCAATGATATTGCA
|
tet(S)
|
tetS-FW
|
ATCAAGATATTAAGGAC
|
573
|
55
|
[83]
|
tetS-RV
|
TTCTCTATGTGGTAATC
|
tet(T)
|
TetT-FW
|
AAGGTTTATTATATAAAAGTG
|
169
|
46
|
[83]
|
TetT-RV
|
AGGTGTATCTATGATATTTAC
|
tet(W)
|
tetWI
|
GGMCAYRTGGATTTYWTIGC
|
1,187
|
64
|
[83]
|
tetWII
|
TCIGMIGGIGTRCTIRCIGGRC
|
van(A)
|
vanA-36F
|
TTGCTCAGAGGAGCATGACG
|
957
|
65
|
[9]
|
vanA-992R
|
TCGGGAAGTGCAATACCTGC
|
van(B)
|
vanB-23F
|
TTATCTTCGGCGGTTGCTCG
|
994
|
62
|
[9]
|
vanB-1016R
|
GCCAATGTAATCAGGCTGTC
|
hdc
|
CL1
|
CCWGGWAAWATWGGWAATGGWTA
|
500
|
48
|
[84]
|
JV17HC
|
AGACCATACACCATAACCTT
|
ldc
|
CAD2-F
|
CAYRTNCCNGGNCAYAA
|
1,185
|
53
|
[82]
|
CAS2-R
|
GGDATNCCNGGNGGRTA
|
odc
|
3
|
GTNTTYAAYGCNGAYAARACNTAYTTYGT
|
1,446
|
52
|
[86]
|
16
|
TACRCARAATACTCCNGGNGGRTANGG
|
tdc
|
TD5
|
CAAATGGAAGAAGAAGTAGG
|
1,100
|
48
|
[81]
|
TD2
|
ACATAGTCAACCATRTTGAA
|
ERIC-PCR
|
ERIC-1R
|
ATGTAAGCTCCTGGGGATTCAC
|
variable
|
46
|
[33]
|
ERIC-2
|
AAGTAAGTGACTGGGGTGAGCG
|
rep-PCR
|
(GTG)5
|
GTGGTGGTGGTGGTG
|
variable
|
40
|
[36]
|
a K=G or T; R=A or G; W=A or T; Y=C or T; S=C or G; M=A or C; D=A, G, or T; N=A, G, C, or T |
