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Supplementary material

LINE1 methylation in granulocyte DNA and trihalomethane exposure on bladder cancer risk
Lucas A. Salas 1,2,3 / Cristina M. Villanueva 1,2,3,4 / Salman M. Tajuddin 5/ André F. S. Amaral 5 / Agustín F. Fernandez 6 / Lee E. Moore 7/ Alfredo Carrato 8,9/ Adonina Tardón 3,10/ Consol Serra 11,12/ Reina García-Closas 13/ Xavier Basagaña 1,2,3 /Nathaniel Rothman 7 / Debra T. Silverman 7/ Kenneth P. Cantor 7/ Manolis Kogevinas 1,2,3,4,14/ Francisco X. Real 2,15/ Mario F. Fraga 6 /Núria Malats 5

1. Centre for Research in Environmental Epidemiology (CREAL), Barcelona, Spain / 2. Universitat Pompeu Fabra (UPF), Barcelona, Spain / 3. CIBER Epidemiología y Salud Pública (CIBERESP), Barcelona, Spain / 4. IMIM (Hospital del Mar Medical Research Institute) / 5. Genetic and Molecular Epidemiology Group, Spanish National Cancer Research Centre (CNIO), Madrid, Spain / 6. Cancer Epigenetics Laboratory, Instituto Universitario de Oncología del Principado de Asturias (IUOPA-HUCA), University of Oviedo, Oviedo, Spain / 7. National Cancer Institute, Bethesda, USA / 8. University Hospital of Elche, Elche, Spain / 9. Hospital Ramon y Cajal, Madrid / 10. University of Oviedo, Oviedo, Spain / 11. Universitat Pompeu Fabra (UPF). Centre of Research in Occupational Health, Spain / 12. Consorci Hospitalari Parc Taulí, Sabadell, Spain / 13. Hospital Universitario de Canarias, La Laguna, Spain / 14. National School of Public Health, Athens, Greece / 15. Epithelial Carcinogenesis Group, Spanish National Cancer Research Centre (CNIO), Spain.

Address correspondence to C.M. Villanueva, CREAL- Centre for Research in Environmental Epidemiology, Barcelona Biomedical Research Park, Dr. Aiguader, 88, 08003 Barcelona, Spain. Telephone: (34) 93 2147300. Fax: (34) 932147302. E-mail: cvillanueva@creal.cat. N. Malats, Genetic and Molecular Epidemiology Group, Spanish National Cancer Research Centre (CNIO), Melchor Fernández Almagro, 3, 28029, Madrid, Spain. Telephone: (34) 912246900. Fax (34) 912246980 E-mail: nmalats@cnio.es

Table of contents


Supplementary Material, Figure S2. Scatterplots of the first and second principal component from a principal component analysis of the 4 CpGs evaluated, according to different variables to test batch effects. 5




Supplementary Material, Figure S1. Distribution of LINE1 %5-MeC, in average and by the four CpG in cases and controls.


Supplementary Material, Figure S2. Scatterplots of the first and second principal component from a principal component analysis of the 4 CpGs evaluated, according to different variables to test batch effects.



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