Supplementary Table fish analysis for 22 patients with informative cytogenetics




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títuloSupplementary Table fish analysis for 22 patients with informative cytogenetics
fecha de publicación27.02.2016
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Supplementary Table 9. FISH analysis for 22 patients with informative cytogenetics. Patient number, targeted cytogenetic abnormality, Vysis FISH probe used, number of FISH+ cells (FISH+ defined by leukemic abnormality) per number of events counted (with percentage), white blood cell count (WBC), derived leukemic cells and derived non-leukemic cells (percentage leukemic or non-leukemic cells multiplied by WBC) are shown for each day (D).

Patient

Cytogenetic abnormality

FISH probe

D

FISH+/No. counted (%)

WBC x 109/L

Absolute leukemia cell count / μL

Absolute non-leukemia cell count / μL

2

47,XY,+8,t(9;22) (q34;q11.2),i(17)(q10)

Chr.8 centromere

-9

312/500 (62.4)

6.2

3869

2331


-8

342/500 (68.4)

7.9

5404

2496


-7

423/500 (84.6)

12.3

10406

1894


-6

630/800 (78.8)

20.4

16075

4325


-3

331/500 (66.2)

12.1

8010

4090

3

47,idem,+14

TelVysion 14q

-9

57/500 (11.4)

3.7

422

3278


-8

70/500 (14.0)

5.1

714

4386


-7

85/500 (17.0)

9.0

1530

7470


-6

87/500 (17.4)

8.0

1392

6608


-3

52/500 (10.4)

9.3

967

8333

5

45,XX,del(5)(q15q33),

-7,+8,-12,-15,-18,+2mar

Chr.8 centromere

-9

49/400 (12.3)

3.2

394

2806


-8

70/400 (17.5)

6.4

1120

5280


-7

77/400 (19.2)

9.3

1786

7514


-6

74/400 (18.5)

13.0

2405

10595


-3

32/400 (8.0)

12.5

1000

11500

6

47,XY,+8

Chr.8 centromere

-9

18/400 (4.5)

0.4

18

382


-8

47/400 (11.8)

0.6

71

529


-7

51/400 (12.8)

0.9

115

785


-6

55/400 (13.8)

7.6

1049

6551


-3

16/400 (4.0)

16.1

644

15456

7

46,XY,del(5)(q13q33),

-7,+mar

Chr.7 centromere

-9

26/400 (6.5)

1.9

124

1777


-8

48/400 (12.0)

4.3

516

3784


-7

58/400 (14.5)

9.0

1305

7695


-6

38/400 (9.5)

16.7

1587

15114


-3

23/400 (5.7)

20.9

1191

19709

8

47,XX,+8

Chr.8 centromere

-9

35/400 (8.8)

2.9

255

2645


-8

41/400 (10.3)

5.3

546

4754


-7

43/400 (10.8)

9.8

1058

8742


-6

48/400 (12.0)

55.6

6672

48928


-3

20/400 (5.0)

47.0

2350

44650

12

45,XY,-7

Chr.7 centromere

-9

23/400 (5.8)

5.8

334

5467


-8

26/400 (6.5)

6.4

416

5984


-7

31/400 (7.8)

17.1

1325

15775


-6

49/400 (12.3)

20.1

2462

17638


-3

25/400 (6.3)

11.6

725

10875

15

Diploid XX

(Diploid XY post-transplant)

CEPX(red)/Y(green)

-9

2/400 (0.5)

2.3

12

2289


-8

2/400 (0.5)

3.7

19

3682


-7

2/400 (0.5)

6.9

35

6866


-6

42/800 (5.3)

9.7

514

9186


-3

29/800 (3.6)

8.5

306

8194

17

47,XX,+8

Chr.8 centromere

-9

21/400 (5.3)

1.4

74

1327


-8

31/400 (7.8)

1.3

101

1199


-7

50/400 (12.5)

1.9

238

1663


-6

145/800 (18.1)

2.7

490

2210


-3

129/800 (16.1)

2.4

387

2013

19

46,XX,del(7)(q22q34)

D7Z1/7q31

(Dual color probe)

-9

130/400 (32.5)

1.6

520

1080


-8

112/300 (37.3)

1.8

671

1129


-7

216/400 (54.0)

1.8

972

828


-6

168/400 (42.0)

2.8

1176

1624


-3

116/400 (29.0)

1.5

435

1065

20

45-46,XX,-9, t(11;19)(q23;p13),+mar

Chr.9 centromere

-9

37/200 (18.5)

3.7

685

3016


-8

39/200 (19.5)

9.1

1775

7326


-7

64/200 (32.0)

18.6

5952

12648


-6

67/200 (33.5)

32.2

10787

21413


-3

45/200 (22.5)

48.6

10935

37665

21

46,XX,t(9;22)(q34;q11.2)

BCR/ABL

(Dual color probe)

-9

60/200 (30.0)

2.4

720

1680


-8

58/200 (29.0)

6.6

1914

4686


-7

77/200 (33.5)

8.8

2948

5852


-6

82/200 (41.0)

10.6

4346

6254


-3

51/200 (25.5)

20.3

5177

15124

22

46,XX,-4,-6,

inv(9)(p12q13),+2mar

Chr.6 centromere

-9

49/200 (24.5)

0.7

172

529


-8

67/200 (33.5)

0.7

235

466


-7

98/200 (49.0)

1.5

735

765


-6

146/400 (36.5)

1.5

548

953


-3

61/200 (30.5)

0.5

153

348

27

46,XY,inv(9)(p12q13),del(20)(q11.2q13.3)

20q12

-9

41/300 (13.7)

1.0

137

863


-8

66/300 (22.0)

4.1

902

3198


-7

87/300 (29.0)

11.7

3393

8307


-6

114/300 (38.0)

26.6

10108

16492


-3

50/300 (16.7)

38.1

6363

31737

29

47,XX,+8

Chr.8 centromere

-9

15/300 (5.0)

3.6

290

5510


-8

61/300 (20.3)

5.8

1177

4623


-7

80/300 (26.7)

27.2

7262

19938


-6

94/300 (23.5)

37.8

8883

28917


-3

25/300 (8.3)

62.5

5188

57313

30


43,XY,del(5)(q11.2q15),

-7,add(7)(q22),t(9;12)

(q22;p13),-16,-17,-20,






Chr.7 centromere

-9

36/300 (12.0)

6.3

756

5544


-8

40/300 (13.3)

16.5

2195

14306


-7

92/300 (23.0)

22.4

5152

17248


-6

76/300 (25.3)

32.3

8172

24128


-3

35/300 (11.7)

63.1

7383

55717

31


46,XX,del(5)(q13q33)






EGR1(5q31)/5p15.2

(Dual color probe)

-9

39/300 (13.0)

2.7

351

2349


-8

67/300 (22.3)

2.3

513

1787


-7

130/300 (43.3)

3.0

1299

1701


-6

210/300 (70.0)

16.3

11410

4890


-3

213/300 (71.0)

11.2

7952

3248

32


44,XX,t(2;3),-4,+8,-16,-17






Chr.8 centromere

-9

7/300 (2.3)

5.8

133

5667


-8

14/300 (4.7)

12.0

564

11436


-7

21/300 (7.0)

24.8

1736

23064


-6

30/300 (10)

41.1

4110

36990


-3

15/300 (5)

55.4

2770

52630

36


46,XY,del(5)(q31q33),del(7)

(q22q34),-11,-13,del(15)

(q21),-16,del(18)(q21),

del(20)(q11.2q13.3),add(21)






11q23

-9

83/300 (27.8)

3.0

834

2166


-8

89/300 (29.7)

3.6

1069

2531


-7

130/300 (43.3)

4.3

1862

2438


-6

148/400 (37.0)

7.1

2627

4473


-3

84/300 (28.0)

3.3

924

2376

37

50,XX,del(5)(q11.2q35),+7,

del(7)(q11.2),der(7)t(5;7)

(p13;p11.2)del(7)(7q11. 2)

x4,del(11)(p11.2p15)

Chr.7 centromere

-9

15/300 (5.0)

1.8

90

1710


-8

13/200 (6.5)

4.4

286

4114


-7

29/300 (9.7)

7.2

698

6502


-6

54/500 (10.8)

7.3

788

6512


-3

16/300 (5.3)

40.5

2147

38354

39

45,XX,t(5;13)(q15;q14),

t(11;20)(q13;q11.2),

-12,add(15)(p12)

12p13

(Dual color probe)

-9

37/300 (12.3)

4.0

492

3508


-8

40/300 (13.3)

27.6

3671

23929


-7

50/300 (16.7)

43.1

7198

35902


-6

59/300 (19.7)

62.0

12214

49786


-3

34/300 (11.3)

100.3

11334

88966

41

46,XY,del(12)(p12p13)

12p13

(Dual color probe)

-9

167/300 (55.7)

2.6

1448

1152


-8

185/300 (61.7)

2.8

1728

1072


-7

219/300 (73.0)

2.3

1679

621


-6

237/300 (79.0)

3.8

3002

798


-3

165/300 (55.0)

3.5

1925

1575

43

46,XX,t(9;22)(q34;q11.2)

BCR/ABL

(Dual color probe)

-9

11/200 (5.5)

12.5

688

11813


-8

16/200 (8.0)

35.9

2872

33028


-7

23/200 (11.5)

65.0

7475

57525


-6

27/200 (13.5)

74.3

10031

64270


-3

14/200 (7.0)

55.0

3850

51150

44

47,XY,del(4)(q31),-20,+2mar

20q13.2

-9

n/a

1.8

n/a

n/a


-8

47/300 (15.7)

3.8

597

3203


-7

78/300 (26.0)

6.8

1768

5032


-6

72/300 (24.0)

10.6

2544

8056


-3

52/300 (17.3)

23.8

4117

19683

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